High Performance Liquid Chromatography Testing Quick Discussion

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HPLC is an exceptionally helpful Kind of substance testing that scientists frequently use to different components in a mix, identify what they are, and determine how much of each is present in a solution. The testing involves pumping a pressurized liquid solvent which contains the sample liquid through a column filled with a sound, adsorbent material. The atoms, ions or molecules of each component in the sample solution will interact differently with the adsorbent, finally causing the elements to different as they flow from the column. Once separated, we can identify and gauge the components. HPLC analysis of polymers other than proteins is infrequent and very challenging, says Jason Todd, our chromatography laboratory manager and expert. The selection of solvents that may dissolve a polymer is typically very limited, compared to small molecules, and restricts the sort of HPLC method you can use.

Also, polymers have a distribution of chain lengths, which frequently will lead to several peaks or a very broad peak during the HPLC separation. We are in need of one, fairly narrow summit to measure the amount of each polymer that is present. Reaching one, narrow peak for every polymer needs a whole lot of method development concerning finding the ideal hplc testing column and mobile phase solvents. Recently, Polymer Solutions took on a project that had stymied two labs. The customer asked PSI to measure preservative compounds parabens within a water-based cleaner that comprised a polymer. In initial tests, the polymer did not behave as expected and a few of the preservative compounds were not very water-soluble, so they did not respond well in HPLC testing.

After discussing with the Customer performance demands and what previous testing other labs had attempted, PSI’s team gathered information about the composition of the test sample and the properties of the polymers, such as molecular weight, chemical composition and solubility. A literature review turned up helpful testing information for one of the polymers, but nothing on the other. At the time we were Beginning the procedure development, I was lucky enough to be attending a technical seminar dealing with plastic analysis, and I was able to gain valuable insight by attending presentations and speaking with leading specialists in the area of polymer HPLC separations, Jason says. Our team used orthogonal HPLC methods to do initial screening experiments. We chose one HPLC way of additional development, based on its first performance in separating the goal polymers. We refined the procedure parameters until we could attain optimum results from the testing.